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ORIGINAL ARTICLE
Year : 2019  |  Volume : 14  |  Issue : 5  |  Page : 448-458

In vitro cytotoxicity assay of D-limonene niosomes: an efficient nano-carrier for enhancing solubility of plant-extracted agents


1 Department of Clinical Biochemistry, Faculty of Medicine; Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, I.R. Iran
2 Department of Chemistry, Shahid Bahonar University of Kerman, Kerman, I.R. Iran
3 Research Center of Advanced Technologies in Medicine, Torbat Heydariyeh University of Medical Sciences, Ttorbat Heydariyeh, I.R. Iran
4 Department of Clinical Biochemistry, Afzalipoor Faculty of Medicine, Kerman University of Medical Sciences, Kerman, I.R. Iran
5 Department of Biotechnology, Institute of Science, High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, I.R. Iran

Correspondence Address:
Masoud Torkzadeh-Mahani
Department of Biotechnology, Institute of Science, High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman
I.R. Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1735-5362.268206

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The low solubility of the plant-extracted agent like D-limonene in cancer therapy is a critical problem. In this study, we prepared D-limonene-loaded niosomes (D-limonene/Nio) for cancer therapy through in vitro cytotoxicity assay of HepG2, MCF-7, and A549 cell lines. The niosomal formulation was prepared by film hydration technique with Span® 40: Tween® 40: cholesterol (35:35:30 molar ratio) and characterized for vesicle distribution size, morphology, entrapment efficiency (EE%), and in vitro release behaviour. The obtained niosomes showed a nanometric size and spherical morphology with EE% about 87 ± 1.8%. Remarkably prolonged release of D-limonene from niosomes compared to free D-limonene observed. The loaded formulation showed significantly enhanced cytotoxic activity with all three cancer cell lines (HepG2, Macf-7 and A549) at the concentration of 20 μM. These results indicated that niosome loaded with phytochemicals can be a promising nano-carrier for cancer therapy applications


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