Research in Pharmaceutical Sciences

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 14  |  Issue : 3  |  Page : 209--215

Differentiation of adult human mesenchymal stem cells into dopaminergic neurons


Marjan Khademizadeh1, Manoochehr Messripour1, Nazem Ghasemi2, Fariborz Momen beik3, Ahmad Movahedian Attar1 
1 Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran
2 Department of anatomical sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran
3 Department of Chemistry, School of Chemistry, University of Isfahan, Isfahan, I.R. Iran

Correspondence Address:
Ahmad Movahedian Attar
Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan
I.R. Iran

The striatal dopamine (DA) deficiency is known as the main cause of the clinical picture of Parkinson’s disease (PD). The disease is a progressive degeneration of dopaminergic neurons in the striatum. The treatment of PD is based on compensation for the brain’s supply of DA lost by drug therapy, deep brain stimulation, surgery, gene and cell therapies. Clinical studies have focused on the utility of stem cell-based therapies in PD. Embryonic and mesenchymal stem cells (MSCs) are widely used. Recently, human adipose derived stem cells (hADSCs) have been considered as a suitable source of tissue for this purpose. In this project, hADSCs differentiated into dopaminergic neurons and the specificity of the cell preparations was examined. Human adipose tissues were collected from healthy volunteers undergoing liposuction and hADSCs were isolated by collagenase-based enzymatic method. Flow cytometry was performed using the surface cluster of differentiation (CD) markers to confirm the cell typical properties. Then hADSCs were differentiated to dopaminergic neurons in neurobasal medium in the presence of differentiation factors and confirmed by immunocytochemistry via neuronal and dopaminergic markers. The isolated hADSCs were cultured and identified by the expression of MSCs surface markers including CD90, and CD44. These cells did not express hematopoietic surface markers such as CD45 and CD14. Differentiated cells express neuronal marker NeuN and dopaminergic marker tyrosine hydroxylase (TH). It is concluded that hADSCs can be easily taken from the patient’s own body and differentiated into dopaminergic cells having a lower risk of transplant rejection.


How to cite this article:
Khademizadeh M, Messripour M, Ghasemi N, Momen beik F, Movahedian Attar A. Differentiation of adult human mesenchymal stem cells into dopaminergic neurons.Res Pharma Sci 2019;14:209-215


How to cite this URL:
Khademizadeh M, Messripour M, Ghasemi N, Momen beik F, Movahedian Attar A. Differentiation of adult human mesenchymal stem cells into dopaminergic neurons. Res Pharma Sci [serial online] 2019 [cited 2019 Nov 19 ];14:209-215
Available from: http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2019;volume=14;issue=3;spage=209;epage=215;aulast=Khademizadeh;type=0