Figure 4: CdCl2 induced the expression of progesterone receptors (PR) in an ER-dependent manner. The expression of (A) PRA and (B) PRB in the presence of ICI 182,780 and without ICI 182,780 were assayed using western blotting method in CdCl2-treated cells. Quantitative analysis of the expression of PRA in (C) OVCAR3, (D), SKOV3 cells; PRB in (E) OVCAR3, and (F) in SKOV3 cells were performed by image j software. Asterisks show significant differences between CdCl2-treated cells in the presence of ICI 182,780 and corresponding cells without ICI 182,780 (*P < 0.05, **P < 0.01, and *** P < 0.001). CC, CdCl2; ICI, ICI 182,780.