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  Indian J Med Microbiol
 

Figure 3: Pathological analysis of brain sections. Group A, RAPA + HSO/EPO-treated mice showed (A1) a large number of inflammatory cells, strings of the non-myelin axon, neuronophagia, (A2) vacuolation, spongy lesions, and (A3) extensive demyelination; group B, rapamycin-treated mice showed (B1) infiltration of inflammatory cells, (B2) spongiotic zones, and (B3) demyelination; group C, HSO/EPO-treated mice showed (C1), a few inflammatory cells and (C2,3) without spongy lesions and demyelination; group D, EAE mice showed (D1) extensive focal inflammatory cells, (D2) extensive vacuolation, zones of spongy degeneration, and (D3) demyelination; group E1-3, the section of the brain of naive mice exhibited no clinical signs. The first row was stained with H&E, the second and third rows were stained with LFB; F, histological score: 0 = no pathology, 1 = no tissue damage but minor inflammation, 2 = modest inflammation, prime tissue damage and demyelination, 3 = moderate tissue damage (demyelination, neuronal loss, tissue damage, cell death, neuronal vacuolation, and neuronophagia), 4 = necrosis (loss of all tissue elements entirely with associated cellular remains). Data presented as mean ± SEM. * P value ≤ 0.05 is significant. EAE, experimental autoimmune encephalomyelitis; HSO/EPO, hemp seed oil/evening primrose oil; RAPA, rapamycin; H&E, hematoxylin and eosin; LFB, luxol fast blue.

Figure 3: Pathological analysis of brain sections. Group A, RAPA + HSO/EPO-treated mice showed (A<sub>1</sub>) a large number of inflammatory cells, strings of the non-myelin axon, neuronophagia, (A<sub>2</sub>) vacuolation, spongy lesions, and (A<sub>3</sub>) extensive demyelination; group B, rapamycin-treated mice showed (B<sub>1</sub>) infiltration of inflammatory cells, (B<sub>2</sub>) spongiotic zones, and (B<sub>3</sub>) demyelination; group C, HSO/EPO-treated mice showed (C<sub>1</sub>), a few inflammatory cells and (C<sub>2,3</sub>) without spongy lesions and demyelination; group D, EAE mice showed (D<sub>1</sub>) extensive focal inflammatory cells, (D<sub>2</sub>) extensive vacuolation, zones of spongy degeneration, and (D<sub>3</sub>) demyelination; group E<sub>1-3</sub>, the section of the brain of naive mice exhibited no clinical signs. The first row was stained with H&E, the second and third rows were stained with LFB; F, histological score: 0 = no pathology, 1 = no tissue damage but minor inflammation, 2 = modest inflammation, prime tissue damage and demyelination, 3 = moderate tissue damage (demyelination, neuronal loss, tissue damage, cell death, neuronal vacuolation, and neuronophagia), 4 = necrosis (loss of all tissue elements entirely with associated cellular remains). Data presented as mean ± SEM. * <i>P</i> value ≤ 0.05 is significant. EAE, experimental autoimmune encephalomyelitis; HSO/EPO, hemp seed oil/evening primrose oil; RAPA, rapamycin; H&E, hematoxylin and eosin; LFB, luxol fast blue.