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  Indian J Med Microbiol
 

Figure 3: Intracellular reactive oxygen species (ROS) level in galectin-9 (Gal-9) treated OVCAR-3 cells. The cells were incubated with various concentrations of Gal-9 (0, 1, 10, 50, and 100 nM) for 48 h and the ROS levels monitored by DCFH-DA staining. The fluorescence intensity was analyzed by a microplate reader at an excitation wavelength of 485 nm and an emission wavelength of 528 nm, respectively. *P < 0.05 is considered significant.

Figure 3: Intracellular reactive oxygen species (ROS) level in galectin-9 (Gal-9) treated OVCAR-3 cells. The cells were incubated with various concentrations of Gal-9 (0, 1, 10, 50, and 100 nM) for 48 h and the ROS levels monitored by DCFH-DA staining. The fluorescence intensity was analyzed by a microplate reader at an excitation wavelength of 485 nm and an emission wavelength of 528 nm, respectively. *<i>P</i> < 0.05 is considered significant.