Figure 5: Effects of galectin-9 (Gal-9) on mitochondrial membrane potential (ΔΨm), Bcl-2 and Bax expression in human ovarian OVCAR-3 cells. A, after incubation of cells with different concentrations of Gal-9 for 48 h, cells were loaded with JC-1 dye and the potential-dependent accumulation in the mitochondria measured directly. B, cells were treated with different concentration of Gal-9 and then Bcl-2 and Bax expressions were analyzed by western blots using the corresponding specific antibodies. Intensity of each band, C, Bcl2 and D, bax was estimated by imageJ software. Equal sample loadings were confirmed by GAPDH band manner compared with untreated cells (*P < 0.05).