Figure 1: MIN6 cells express A3AR. Total RNA was extracted from MIN6 cells and after cDNA synthesis specific amplicon of A3AR was extended using RT-PCR. (a) Amplification plot in linear form, (b) melt curve, and (c) gene expression levels of A3AR, in comparison with A1AR are presented. Comparison between A1 and A3 receptor expression levels was performed based on 2-ΔΔCT formula. This assay was duplicated in three independent time (n = 6). (*) ΔRn is the value obtained from subtracting of Rn from the baseline: (ΔRn = Rn - baseline), where Rn is the ratio of the emitted fluorescence intensity of the Sybr green to the emitted fluorescence intensity of the passive reference dye (ROX). (**) Derivative reporter (-Rn ′) is the maximum rate of fluorescence changes following the ramping temperature. A3AR, A3 adenosine receptor.