Figure 2: MIN6 cells express an active form of A3AR. Cells were pretreated with Ro-20-1724 (100 μM) for 15 min and then, forskolin (10 μM) for 10 min. Next, Cl-IBMECA (0.1-100 μM) was added to cells for 15 min. Then, cAMP content of each treatment was assayed using specific ELISA. EC50 value was calculated using Graphpad Prism software. This assay was duplicated three times (n = 6) and results were presented as mean ± SD. A3AR, A3 adenosine receptor; Ro-20-1724, a phosphodiesterase inhibitor; Cl-IBMECA, A3AR agonist; EC50, the half maximal effective concentration.