Figure 3: A3AR mediated elevation of intracellular Ca2+ in MIN6 cells. Fura 2/AM (5 μM) was loaded to cells for 1 h and then these cells were stimulated with Cl-IBMECA (1 μM) for 8 min. (a) Fura 2 was stimulated at 340/510 and 380/510 nm to distinguish between Ca2+ bound and free forms of Fura 2, respectively. (b) 340/380 Ratio has been evaluated to indicate elevation of intracellular Ca2+ following Cl-IBMECA treatment. These assays were triplicated in three independent evaluations (n = 9) and results presented as mean ± SD. A3AR, Adenosine receptor; Cl-IBMECA, A3AR agonist.