Figure 4: Cl-IBMECA potentiates GSIS in MIN6 cells. GSIS induced by elevation of glucose levels (from 2.5 mM to 16.5 mM) and then effects of agonists on insulin secretion have been evaluated. MRS 1220 was used as an A3AR antagonist and pretreated at 1μM prior to adding Cl-IBMECA. Insulin content of each treatment was measured using ELISA. This experiment was duplicated in three independent repeats (n = 6) and results were presented as mean ± SD. *P value < 0.05 in comparison with control group. AR, Adenosine receptor; Cl-IBMECA, A3AR agonist; GSIS, glucose stimulated insulin secretion.