Figure 2: Effect of A. indica extract on cholesterol uptake and cell viability in differentiated Caco-2 cells. (A) Cells were incubated with different concentration of A. indica extract at 12.5, 25, 50, 75, and 100 μg/mL or 40 μg/mL of ezetimibe for 3 h at 37 °C. The radioactivity of [3H]-micelle cholesterol was measured and expressed as percent of control. Viability of Caco-2 cells after exposure to either A. indica extract or ezetimibe for (B) 3 h, (C) 24 h, and (D) 48 h was determined using MTT assay. Each experiment was performed separately, n = 5. *** Indicates significant differences compared with control (P < 0.001). A. indica, Azadirachta indica.