Figure 3: NPC1L1 expression in 100 ug of whole cell, 50 ug of membrane, and 50 ug of cytosolic fractions extracted from Caco-2 cells. Cells were incubated with 100 ug/mL of A. indica for 3 h. NPC1L1 proteins were detected using anti-NPC1L1 antibody. ALP and anti-β-actin antibodies were also used as apical membrane marker and loading control, respectively. Data are expressed as mean ± SEM, n = 5. (A) A representative blot of NPC1L1 and β-actin protein expressions and (B) indicated quantification of relative NPC1L1/β -actin. * Shows significant differences compared to control, P < 0.05. NPC1L1, niemann-pick C1 like 1; A. indica, Azadirachta indica; ALP, anti-alkaline phosphatase.