Figure 4: Effect of A. indica extract on PPAR d mRNA expression in Caco-2 cells. Cells were incubated with 100 μg/mL of A. indica extract for 3 h. Total RNAs were extracted from Caco-2 cells and PPAR d mRNA level was determined using quantitative polymerase chain reaction. Data are expressed as mean ± SEM from 3 separate experiments. ** Indicates significant differences compared to control, P < 0.01. A. indica, Azadirachta indica; PPAR, peroxisome proliferator-activated receptor.