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  Indian J Med Microbiol
 

Figure 4: (a) 15% SDS-PAGE analysis of the purification process IL24-BR2 fusion protein, (b). 15% SDS-PAGE analysis of the purification process IL24 protein. Lane 1, protein marker; lane 2, un-induced E. coli BL21 (DE3) containing recombinant pET28a; lane 3, induced E. coli BL21 (DE3) containing recombinant pET28a; lane 4, sample after sonication and centrifuging; lane 5, sample after loading protein to column; lane 6 sample after binding buffer; lane 7, sample after washing buffer; lane 8, sample after elution buffer, and lane 9, sample after dialyze. SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; IL, interleukin; E. coli, Escherichia coli.

Figure 4: (a) 15% SDS-PAGE analysis of the purification process IL24-BR2 fusion protein, (b). 15% SDS-PAGE analysis of the purification process IL24 protein. Lane 1, protein marker; lane 2, un-induced <i>E. coli</i> BL21 (DE3) containing recombinant pET28a; lane 3, induced <i>E. coli</i> BL21 (DE3) containing recombinant pET28a; lane 4, sample after sonication and centrifuging; lane 5, sample after loading protein to column; lane 6 sample after binding buffer; lane 7, sample after washing buffer; lane 8, sample after elution buffer, and lane 9, sample after dialyze. SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; IL, interleukin; <i>E. coli</i>, <i>Escherichia coli</i>.