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  Indian J Med Microbiol
 

Figure 5: Western blot analysis of purified IL24-BR2 and IL24. Lane 1, pre-stained protein marker; lane 2, un-induced E. coli BL21 (DE3) without pET28a; and lane 3, purify IL24 protein after dialyze; lane 4, purify IL24 protein before dialyze; lane 5, induced E. coli BL21 (DE3) containing pET28a-IL24; lane 6, purify IL24-BR2 fusion protein after dialyze, lane 7, purify IL24-BR2 fusion protein before dialyze, and lane 8, induced E. coli BL21 (DE3) containing pET28a-IL24-BR2. IL, Interleukin; E. coli, Escherichia coli.

Figure 5: Western blot analysis of purified IL24-BR2 and IL24. Lane 1, pre-stained protein marker; lane 2, un-induced <i>E. coli</i> BL21 (DE3) without pET28a; and lane 3, purify IL24 protein after dialyze; lane 4, purify IL24 protein before dialyze; lane 5, induced <i>E. coli</i> BL21 (DE3) containing pET28a-IL24; lane 6, purify IL24-BR2 fusion protein after dialyze, lane 7, purify IL24-BR2 fusion protein before dialyze, and lane 8, induced <i>E. coli</i> BL21 (DE3) containing pET28a-IL24-BR2. IL, Interleukin; <i>E. coli</i>, <i>Escherichia coli</i>.