Figure 1: Verification of pCDNA/IL-5 insertion. (A) Genomic DNA amplification. Agarose gel electrophoresis results illustrated the insertion of IL-5 gene into the genome of transfected cells; Lane 1, untransfected cells; lane 2, DNA marker (1 kb Fermentas); and lane 3, amplified fragment of transfected cells (618 bp). (B and C) Expression level of IL-5 protein on HEK-293T cell surface. Flow cytometric analysis shows 76% expression of IL-5 protein on (C) the transfected cells compared to (B) the un-transfected cells.