Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
  • Users Online: 28
  • Home
  • Print this page
  • Email this page


 
 Table of Contents  
ORIGINAL ARTICLE
Year : 2021  |  Volume : 16  |  Issue : 1  |  Page : 94-102

Protective and therapeutic effects of ethanolic extract of Nasturtium officinale (watercress) and vitamin E against bleomycin-induced pulmonary fibrosis in rats


1 Department of Toxicology, Shahreza Branch, Islamic Azad University, Shahreza, I.R. Iran
2 Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, I.R. Iran
3 Clinical Education Research Center, Department of Pathology, Medical School, Shiraz University of Medical Sciences, Shiraz, I.R. Iran

Date of Submission23-Mar-2020
Date of Decision15-Jul-2020
Date of Acceptance13-Dec-2020
Date of Web Publication30-Dec-2020

Correspondence Address:
Hossein Sadeghi
Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj
I.R. Iran
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1735-5362.305192

Rights and Permissions
  Abstract 


Background and purpose: Pulmonary fibrosis is a chronic disease of the lungs caused by inflammation, species of reactive oxygen, and immune defects. Antioxidant properties of Nasturtium officinale has been reported in some studies. Therefore, the objective of the current study was to evaluate the effect of ethanolic extract of Nasturtium officinale (EENO) on bleomycin (BLM)-induced lung fibrosis in rats.
Experimental approach: Forty adult male Wistar rats (180-220 g) were randomly divided into 5 experimental groups. Normal control, BLM control received a single dose of BLM (6 IU/kg) intratracheally only on the first day, EENO + BLM group received EENO (500 mg/kg) one week before intratracheal BLM instillation and two weeks afterward, BLM + EENO group and BML + vitamin E group received EENO (500 mg/kg) and vitamin E (500 mg/kg) half-hour after BLM installation, respectively. The animals were sacrificed on day 22. Change in body weight, lung index, serum level of malondialdehyde (MDA) and nitric oxide (NO) metabolite, lung tissue hydroxyproline content and lung pathology were assessed.
Findings/Results: Pre- or post-treatment with EENO attenuated pulmonary fibrosis as evidenced by normalized lung index, improved histological changes and inhibited collagen deposition (hydroxyproline) in the animal lung. EENO also decreased MDA and NO metabolite release in comparison to the BLM control. vitamin E (500 mg/ kg) also significantly inhibited the BLM-induced lung toxicity.
Conclusions and implications: EENO can prevent BLM-induced lung fibrosis in rats via antioxidant activities. However, more studies are needed to elicit the exact mechanism of this effect.

Keywords: Bleomycin; Fibrosis; Nasturtium officinale; Vitamin E


How to cite this article:
Ramezani S, Javadi I, Kokhdan EP, Omidifar N, Nikbakht J, Sadeghi H, Doustimotlagh AH, Danaei N, Abbasi R, Sadeghi H. Protective and therapeutic effects of ethanolic extract of Nasturtium officinale (watercress) and vitamin E against bleomycin-induced pulmonary fibrosis in rats. Res Pharma Sci 2021;16:94-102

How to cite this URL:
Ramezani S, Javadi I, Kokhdan EP, Omidifar N, Nikbakht J, Sadeghi H, Doustimotlagh AH, Danaei N, Abbasi R, Sadeghi H. Protective and therapeutic effects of ethanolic extract of Nasturtium officinale (watercress) and vitamin E against bleomycin-induced pulmonary fibrosis in rats. Res Pharma Sci [serial online] 2021 [cited 2021 Jan 27];16:94-102. Available from: https://www.rpsjournal.net/text.asp?2021/16/1/94/305192




  Introduction Top


Bleomycin (BLM) as an antibiotic with antitumor, antiviral, and antibacterial activity is commonly used in the treatment of different forms of cancer [1]. Despite the development of new drugs in the treatment of cancer, BLM remains an important drug of chemotherapy regimens for the treatment of some tumors such as germinative tumors and Hodgkin’s lymphoma [2]. BLM hydrolase is mainly responsible for metabolizing BLM to safe molecules. Interestingly, the lung and skin have the lowest concentration of this enzyme. Therefore, these organs are the most important sites of BLM toxicity [3]. BLM-induced pulmonary fibrosis is a fatal adverse effect that has been occurred in up to ten percent of patients receiving the drug [4].

BLM-induced injury to endothelial cells and consequent infiltration of leukocytes weaken pulmonary protective barriers. There are various evidences that point out reactive oxygen species (ROS) and the inflammatory process plays an important role in the pathology and development of pulmonary fibrosis [5]. Accumulated evidence proposed that several factors such as cell proliferation, collagen synthesis, and production and release of cytokines as well as leukotrienes are involved in the induction and progression of pulmonary fibrosis [6]

Emerging evidence has demonstrated that oxidant/antioxidant imbalance is also involved in the pathogenesis of pulmonary fibrosis [7]. At this time, lung transplantation is the only real treatment for pulmonary fibrosis, however, gene therapy has become one of the choices for treatment in the future [8],[9]. Immunosuppressive medications like glucocorticoids are frequently administered in this situation although their impact on patients’ survival and quality of life are uncertain [10]. Therefore, the development of new drugs to prevent or control pulmonary fibrosis is still a problem.

In Iranian folk medicine Nasturtium officinale R. Br. (watercress), from the Brassicaceae family has been broadly used to treatment of a variety of diseases including hypertension, hyperglycemia, and abdominal pain [11]. This is also used to treat other illnesses such as bronchitis, stomach ulcer, pneumonia, influenza, asthma, and diabetes [12]. This plant grows mostly in districts near the river in the spring in Europe and some parts of Asia [13]. This plant’s leaves are commonly eaten in fresh form in salads, soups, and other recipes and are very rich in vitamins, particularly vitamin C [14]. The anticancer, hepatoprotective, nephroprotective, and antihyperlipidemic properties of watercress have been demonstrated in both in vitro and in vivo conditions [15],[16]. Also, some studies have shown that watercress exhibits significant antioxidant and anti-inflammatory activities in experimental conditions [17].

In this context, the current study was designed to evaluate the effects of ethanolic extracts of Nasturtium officinale (EENO), according to the mentioned properties, in the prevention of BLM-induced pulmonary fibrosis in rats compared to vitamin E.


  Material and Methods Top


Thiobarbituric acid, trichloroacetic, hydrochloric acid, chloramine T, sodium acetate, hydroxyproline, sulfanilamide, and N- naphthyl ethylenediamine were purchased from Merck (Darmstadt, Germany). Bleomycin hydrochloride (Bleo-Cell®, 15 mg/vials, Cell Pharm GmbH, Germany) was purchased from a pharmacy.

Animals

Forty healthy adult male Wistar rats (180-220 g g) were purchased from the animal house of Yasuj University of Medical Sciences, Yasuj, I. R. Iran. All the animals were kept under standard laboratory conditions (12/12 h light/dark). The animals had open access to food and tap water and were divided into five groups at random. The experiment was carried out in accordance with the ‘Guide for the Care and Use of Laboratory Animals’ (Ethical code: 19720415942002).

Preparation of extract

Aerial parts of Nasturtium officinale were gathered from Kohgiluyeh and Boyer-Ahmad (Iran) province in May 2017 and authenticated by Dr. A. Jafari (Botany Department, Natural Resource and Animal Husbandry Research Centre, Yasuj University, Yasuj, Iran) and a voucher specimen (Herbarium No. HYU30230) was deposited there.

The air-dried powder of aerial parts (200 g) of the plant was extracted three times with an 1000 mL mixture of ethanol (7:3) at 37 °C for 72 h. The extract was filtered and concentrated by rotary evaporation and dried at 50 °C and then, it was dried at room temperature. The dried extract was weighed and kept at -20 °C for further studies [16].

Experimental design

All animals randomly were divided into five groups, eight each. (1) The control group was given a single dose of normal saline, then received everyday i.p. saline injection; (2) BLM-treated group was only treated intratracheally BLM (6 IU/kg in 0.9% NaCl). Briefly, after anesthesia, the rats were fixed on a board, the tongue was pulled out with forceps, and the fluid was poured at the distal part of the mouth and teeth while the nose was gently closed [18]. (3) EENO + BLM group was given EENO (500 mg/kg, p.o. once a day) before the induction of fibrosis and two weeks after the induction of fibrosis; (4) BLM + EENO group was given EENO (500 mg/kg, p.o., once daily) half an hour after BLM for three weeks; and (5) BLM + vitamin E group was given vitamin E (500 mg/kg, p.o., once a day) for 21 days after BLM challenge [19]. The indicated doses were selected based on our previous studies [17]

Preparation of biochemical and histological samples

On day 22, the body weights of the animals were recorded, then they were euthanized by ether anesthesia and blood samples were collected by cardiac puncture for malondialdehyde (MDA) and nitric oxide (NO) metabolite levels assessment. Then, the lung was carefully removed and weighed. The pulmonary specimens were divided into two sections, one section was submerged in 10% formalin solution for histological analysis and one section was kept in liquid nitrogen for assessment of hydroxyproline level [20].

Assessment of lipid peroxidation

The serum level of MDA was measured according to our previous study [21]. In brief, 375 mg of thiobarbituric acid was diluted in 2 mL of HCl (12 N), followed by 15 g of trichloroacetic acid for a total amount of 100 mL. A half milliliter of serum was then mixed with 2 mL of this solution and heated in a boiling bath of water for 15 min. Finally, the absorbance was read at 535 nm, and the amount of MDA was represented as μmol/mL.

Assessment of hydroxyproline content

The content of hydroxyproline as an index of lung fibrosis in the lung tissues was determined spectrophotometrically based on the previous study [22]. The right lung tissues were weighed and homogenized. Briefly, for digestion, aliquots of 1 mL from the homogenized tissue were mixed with 6 M hydrochloric acid, at 150 °C, for 4 h. After cooling, the solution was neutralized with 6 M sodium hydroxide and filtered. Then, the filtrate was mixed with the solution consisting of 1.4% chloramine T, 10% propanol, and 0.5 M sodium acetate [15], after 20 min Ehrlich’s reagent was added at 65 °C for 15 min. After cooling, absorbance was read at 550 nm and the quantity of hydroxyproline was determined against a standard curve of known concentrations of hydroxyproline.

Assessment of NO metabolite level

Measurement of NO metabolite levels in serum was carried out spectrophotometrically with the Griess method according to our previous study [20]. Briefly, 100 μL of serum was added to the same volume of Griess reagent to generate a purple azo dye. The Griess reagent (1.25% HCl, 5 mg/mL sulfanilamide with 0.25 mg/mL N-naphthyl ethylenediamine) was added at a rate of 0.1 mL/min. The absorbance of the dye output was measured at 540 nm with attention to a standard nitrite curve generated using NaNO2 by Elisa reader.

Histological studies

The rats were euthanized and their lung was carefully removed and fixed in 10% buffered formaldehyde solution for 1 week. Then, the fixed biopsies were embedded in paraffin and cut into 3-4 μm slices (Automatic tissue processor, Auto Technique). The slices were mounted on glass slides and then stained with Hematoxylin-Eosin dye and examined for histopathological evaluation.

Statistical analysis

The collected results were statistically analyzed by SPSS software (Version 17). The results were presented as mean ± SEM and evaluated by a one-way variance analysis (ANOVA) accompanied by Tukey’s post-test. P values less than 0.05 were considered to show significant differences for all comparisons made.


  Results Top


The effect of EENO on bodyweight and lung index

As shown in [Table 1], intratracheal instillation of BLM did not inhibit weight gain but significantly increased lung weight when compared with the normal group (P < 0.05).
Table 1: The effects of BLM, EENO, and Vit E on the body and lung weight. Data are presented as mean ± SEM, n = 6. *P < 0.05 Indicate significant differences compared with the control group

Click here to view


As illustrated in [Figure 1], the lung index in the BLM-treated group was significantly higher than the control group (P < 0.001). Pre- or post- treatment with EENO (500 mg/kg) significantly normalized the increased lung index compared to the BLM-treated group (P < 0.05 and P < 0.001, respectively). Vitamin E (500 mg/kg) also considerably inhibited changes in lung index comparison to BLM- treated rats (P < 0.01).
Figure 1: Effect of EENO extract on lung index of BLM-induced pulmonary fibrosis in rats. Values are presented as mean ± SEM of six independent observations in each group. ***P < 0.001 Indicate significant differences compared with the control group; #P < 0.05, ##PP < 0.01, and ###P < 0.001 vs bleomycin-treated rats. BLM, Bleomycin; EENO, ethanolic extract of Nasturtium officinale; Vit E, vitamin E.

Click here to view


The effect of EENO on hydroxyproline level

As illustrated in [Figure 2], BLM increased the hydroxyproline content in lung tissue in comparison with the control group (P < 0.01). Pre- and post-treatment with EENO (500 mg/kg) significantly decreased the hydroxyproline level when compared to BLM- treated group (P < 0.001 and P < 0.01, respectively). Vitamin E (500 mg/kg) also significantly reduced hydroxyproline content in treated animals compared to the BLM-treated group (P < 0.05).
Figure 2: Effect of EENO extract on HYP content of BLM-induced pulmonary fibrosis in rats. Values are presented as mean ± SEM of six independent observations in each group. **P < 0.01 Indicate significant differences compared with the control group; #P < 0.05, ##PP < 0.01, and ###P < 0.001 vs bleomycin-treated rats. BLM, Bleomycin; EENO, ethanolic extract of Nasturtium officinale; Vit E, vitamin E; HYP, hydroxyproline.

Click here to view


The effect of EENO on NO metabolite and MDA level

As shown in [Figure 3]A and B, BLM caused a significant rise in the serum NO metabolite and MDA level compared with the control group (P < 0.001). Treatment with EENO (500 mg/kg) before and after the BLM challenge inhibited significantly the increase in the serum NO metabolite and MDA level when compared to the BLM-treated group (P < 0.01). Vitamin E (500 mg/kg) also, considerably decreased the serum NO level in treated animals compared to BLM-treated rats (P < 0.01).
Figure 3: Effect of EENO extract on serum levels of MDA and NO of BLM-induced pulmonary fibrosis in rats. Values are presented as mean ± SEM of six independent observations in each group. ***P < 0.001 Indicate significant differences compared with the control group; ##PP < 0.01 and ###P < 0.001 vs bleomycin-treated rats. BLM, Bleomycin; EENO, ethanolic extract of Nasturtium officinale; Vit E, Vitamin E; MDA, malondialdehyde; NO, nitric oxide.

Click here to view


Histopathological assessment

As presented in [Figure 4]A, pathological evaluation of the lung tissue in the normal group showed the well-organized pathological construction of lung tissue without any inflammatory infiltration and interstitial fibrosis. However, the BLM group lung examination revealed that BLM induced inflammatory infiltration, emphysema, and bleeding in the lung that represented the damage of the alveoli [Figure 4]B. EENO (500 mg/kg) treatment considerably reduced inflammatory influx and interstitial fibrosis as compared to the BLM-treated group [Figure 4]D and [Figure 4]E. Treatment with vitamin E is also moderately was able to prevent BLM- induced alterations [Figure 4]C.
Figure 4: The Influence of EENO on histopathological changes of BLM-induced pulmonary fibrosis in rats. (A) Normal lung histology in the saline control; (B) inflammatory cell infiltration and fibrosis in the BLM group; (C) weaker fibrosis in the BLM + vitamin E group (500 mg/kg); (D) reduced lung fibrosis in the EENO + BLM group (500 mg/kg); and (E) marked prevention of fibrosis in the BLM + EENO group (500 mg/kg). BLM, Bleomycin; EENO, ethanolic extract of Nasturtium officinale.

Click here to view



  Discussion Top


Pulmonary fibrosis is a chronic progressive interstitial lung disease as a result of the excessive accumulation of collagen, epithelial cell damage, and disruption of the integrity of alveolar leading to decreasing lung function [23]. BLM-induced pulmonary fibrosis in animals has been developed to better recognize the underlying pathophysiology and examine new components to the treatment of the disorders [24]. It has been well-known that BLM-endotracheal instillation decreases body weight whereas increasing lung weight. These alterations can reduce to some extent with appropriate medication such as antiinflammatory and antioxidants agents [7].

The findings of the current study showed that BLM endotracheal instillation did not prevent the gain of weight but increased lung index in the group treated with BLM compared to the normal group. This finding was consistent with Abidi et al. study [7]. Treatment with EENO and vitamin E considerably reduced the indicated changes induced by BLM. In this line, a previous study reported EENO was able to normalize the ratio of kidney weight/100 mg BW in the vancomycin-induced kidney toxicity in the rat [16].

An essential pathological hallmark of pulmonary fibrosis is extreme deposition collagens, and hydroxyproline is a specific component of collagen hydrolysis. Therefore, lung tissue hydroxyproline content regards as a pulmonary fibrosis mark [25]. The results of the current study indicated that the hydroxyproline level in the lung of BLM-treated animals was considerably higher than that in the control group, whereas the lung hydroxyproline level in the EENO-treated group was considerably lower than that in the BLM group. The subsequent confirmatory pathological study showed significant alveolar space structure alteration in BLM-induced animals with collapsed alveoli, interalveolar inflammation, inflammatory infiltration, and emphysema. Similar histopathological changes have been established by other investigations [25]. This finding proposes that the protection activities of EENO on BLM-induced pulmonary fibrosis were highly associated with reduced lung hydroxyproline levels. In this line, it has been reported that EENO displays potential anti- fibrotic properties in animal models of liver fibrosis [15],[26].

An imbalance between antioxidant agents and free radicals causes oxidative stress, which plays an important role in the pathophysiology of various diseases, including liver and kidney toxicity, inflammation and pulmonary fibrosis [27],[28]. Several data are pointing to the important role of oxidative stress in lung fibrosis pathophysiology [29]. It has been reported that ROSs and reactive nitrogen species rise in animal models of pulmonary fibrosis [30]. NO, an endogenous short-term free radical, is generated by three NO synthase (NOS) isoforms, including neuronal, endothelial, and inducible NOS isoforms [31]. Recent studies have shown that an excessive generation of NO, nitrogen dioxide, and peroxynitrite plays a significant role in the development of pulmonary fibrosis in both human and animal models pulmonary fibrosis [32]. Our results indicated that the serum NO metabolite concentration in the pulmonary fibrosis caused by the BLM was significantly higher than that in the normal group. The rise in nitrite concentration of BLM-treated rats is in accordance with the result of El-Khouly et al. [29]. Kalayarasan et al. reported that BLM augmented iNOS expression in pulmonary fibrosis [33]. The serum level of NO metabolite in the EENO-treated group was significantly lower than that in the BLM-treated group, proposing that EENO could attenuate lung fibrosis via suppressing the generation of RNAs. In this regard, watercress was observed to possess a NO scavenging property in vitro condition, an ability that might be because of its phenolic/flavonoid content [34]. It also reported that watercress produced a noticeable effect on the elevated NO concentration in the other animal models of toxicology [17].

It has been established that ROS generation is associated with the Fe (II) to Fe (III) oxidation. This cycle promotes oxygen reduction to free radicals that participate in BLM-antitumor activity [29]. Intracellular ROSs attack the cell membrane via polyunsaturated fatty acids disintegration, which clarifies in consequence MDA rise subsequent BLM challenge. MDA is a reactive carbon agent that is used as a marker of lipid peroxidation [35]. In line with the previous studies, our data showed that BLM-endotracheal instillation induced a significant increase in the serum levels of MDA in BLM-treated animals. Pre- and post- treatment with EENO similar to vitamin E significantly reduced the increased levels of MDA serum due to BLM endotracheal instillation. These findings indicated that EENO could decrease lung fibrosis by inhibiting oxidative stress pathways.

It is important to mention, that phytochemical evaluation of the Nasturtium officinale extract in our previous study revealed that benzenepropanenitrile (48.3%), phytol (10.1%), a-cadinene (9.5%), and linolenic acid (8.0%) are its main constituents [27]. Furthermore, the existence of phenolic and flavonoid compounds in the total extract of Nasturtium officinale has been quantified. The presence of rutin, chlorogenic, and caffeic acids were also reported in the plant [36]. Nasturtium officinale was identified by some studies for its antioxidant and anti-inflammatory benefits [15],[17],[37]. In this context, these phenolic components in the plant via the antioxidant and anti-inflammatory activities could have a significant role in the attenuating of pulmonary fibrosis.

At last not at least, pre- or post-treatment with EENO lessened the BLM-induced pulmonary, proposing the potential value of EENO as a component to avoid and manage pulmonary fibrosis. Furthermore, the data of the present study indicated the efficacy of EENO on pulmonary fibrosis were similar to those of vitamin E, indicating strong antioxidant activity of the extract.


  Conclusion Top


This study confirmed the anti-fibrotic efficacy of EENO against BLM-induced pulmonary fibrosis in rats. EENO seemed to cause a pneumoprotective effect through the improvement of antioxidant status and reduction in collagen accumulation in lung tissue. These results propose the potential therapeutic properties of Nasturtium officinale for preventing or treatment of pulmonary fibrosis. However, further studies will be needed to clarify the exact mechanisms, finding human dosage, and possible toxicity of EENO in the management of pulmonary fibrosis.

Acknowledgments

We would like to appreciate the staffs of the Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran for their technical support.

Conflict of interest statement

The authors declare no conflict of interest in this study.

Authors’ contribution

The idea was developed by H. Sadeghi and I. Javadi. H. Sadeghi, J. Nikbakht and A.H. Doustimotlagh supervised this work. in vivo investigations were done by E. Panahi Kokhdan, N. Omidifar, and S. Ramezani. Data collection and analysis were performed by E. Panahi Kokhdan, N. Omidifar, S. Ramezani and A.H. Doustimotlagh. H. Sadeghi, N. Danaei. J. Nikbakht and R. Abbasi contributed to manuscript preparation and revision.



 
  References Top

1.
Hsu HS, Liu CC, Lin JH, Hsu TW, Hsu JW, Su K, et al. Involvement of ER stress, PI3K/AKT activation, and lung fibroblast proliferation in bleomycin- induced pulmonary fibrosis. Sci Rep. 2017;7:14272,1-11. DOI:10.1038/s41598-017-14612-5.  Back to cited text no. 1
    
2.
Bik L, Sangers T, Greveling K, Prens E, Haedersdal M, van Doorn M. Efficacy and tolerability of intralesional bleomycin in dermatology: a systematic review. J Am Acad Dermatol. 2020;83(3):888-903. DOI: 10.1016/j.jaad.2020.02.018.  Back to cited text no. 2
    
3.
Zuo WL, Zhao JM, Huang JX, Zhou W, Lei ZH, Huang YM, et al. Effect of bosentan is correlated with MMP-9/TIMP-1 ratio in bleomycin-induced pulmonary fibrosis. Biomed Rep. 2017;6(2):201-205. DOI: 10.3892/br.2016.832.  Back to cited text no. 3
    
4.
Ortiz LA, Gambelli F, McBride C, Gaupp D, Baddoo M, Kaminski N, et al. Mesenchymal stem cell engraftment in lung is enhanced in response to bleomycin exposure and ameliorates its fibrotic effects. Proc Natl Acad Sci. 2003;100(14):8407- 8411. DOI: 10.1073/pnas.1432929100.  Back to cited text no. 4
    
5.
Ma WH, Li M, Ma HF, Li W, Liu L, Yin Y, et al. Protective effects of GHK-Cu in bleomycin-induced pulmonary fibrosis via anti-oxidative stress and anti- inflammation pathways. Life Sci. 2020;241:117139. DOI: 10.1016/j.lfs.2019.117139,1-13.  Back to cited text no. 5
    
6.
Wuyts WA, Agostini C, Antoniou KM, Bouros D, Chambers RC, Cottin V, et al. The pathogenesis of pulmonary fibrosis: a moving target. Eur Respir J. 2013;41(5):1207-1218. DOI: 10.1183/09031936.00073012.  Back to cited text no. 6
    
7.
Abidi A, Kourda N, Feki M, Ben Khamsa S. Protective effect of Tunisian flaxseed oil against bleomycin-induced pulmonary fibrosis in rats. Nutr Cancer. 2020;72(2):226-238. DOI: 10.1080/01635581.2019.1622741.  Back to cited text no. 7
    
8.
Khalvati B, Sheikhsaran F, Sharifzadeh S, Kalantari T, Behzad Behbahani A, Jamshidzadeh A, et al. Delivery of plasmid encoding interleukin-12 gene into hepatocytes by conjugated polyethylenimine- based nanoparticles. Artificial Cells, Nanomed. Biotechnol. 2017;45(5):1036-1344. DOI: 10.1080/21691401.2016.1202256.  Back to cited text no. 8
    
9.
Dehshahri A, Sadeghpour H, Keykhaee M, Khalvati B, Sheikhsaran F. Enhanced delivery of plasmid encoding interleukin-12 gene by diethylene triamine penta-acetic acid (DTPA)-conjugated PEI nanoparticles. Appl Biochem Biotechnol. 2016;179(2):251-269. DOI: 10.1007/s12010-016-1991-1.  Back to cited text no. 9
    
10.
Jo HE, Randhawa S, Corte TJ, Moodley Y. Idiopathic pulmonary fibrosis and the elderly: diagnosis and management considerations. Drugs Aging. 2016;33(5):321-334. DOI: 10.1007/s40266-016-0366-1.  Back to cited text no. 10
    
11.
Yazdanparast R, Bahramikia S, Ardestani A. Nasturtium officinale reduces oxidative stress and enhances antioxidant capacity in hypercholesterolaemic rats. Chem Biol Interact. 2008;172(3):176-184. DOI: 10.1016/j.cbi.2008.01.006.  Back to cited text no. 11
    
12.
Amiri H. Volatile constituents and antioxidant activity of flowers, stems and leaves of Nasturtium officinale R. Br. Nat Prod Res. 2012;26(2):109-115. DOI: 10.1080/14786419.2010.534998.  Back to cited text no. 12
    
13.
Mazandarani M, Momeji A, Zarghami MP. Evaluation of phytochemical and antioxidant activities from different parts of Nasturtium officinale R. Br. in Mazandaran. Iran J Plant Physiol. 2012;3(2):659-664. DOI: 10.22034/IJPP.2013.540676.  Back to cited text no. 13
    
14.
Simmonds MSJ, Howes MJR. Plants used in the treatment of diabetes. In: Soumyanath A, editor. Traditional medicine for modern times-antidiabetic plants. 1st ed. CRC Press; 2006. pp. 19-82.  Back to cited text no. 14
    
15.
Sadeghi H, Azarmehr N, Razmkhah F, Sadeghi H, Danaei N, Omidifar N, et al. The hydroalcoholic extract of watercress attenuates protein oxidation, oxidative stress, and liver damage after bile duct ligation in rats. J Cell Biochem. 2019;120(9):14875-14884. DOI: 10.1002/jcb.28749.  Back to cited text no. 15
    
16.
Karami M, Mostafazadeh M, Sadeghi H, Sadeghi H, Mehraban F, Kokhdan EP, et al. Nephroprotective effect of Nasturtium officinale (watercress) ethanol extract and Vitamin E on vancomycin-induced nephrotoxicity in rats. Jundishapur J Nat Pharm Prod. 2018;13(1):e67178,1-8. DOI: 10.5812/jjnpp.67178.  Back to cited text no. 16
    
17.
Sadeghi H, Mostafazadeh M, Sadeghi H, Naderian M, Barmak MJ, Talebianpoor MS, et al. In vivo antiinflammatory properties of aerial parts of Nasturtium officinale. Pharm Biol 2014;52(2):169-174. DOI: 10.3109/13880209.2013.821138.  Back to cited text no. 17
    
18.
Kandhare AD, Bodhankar SL, Mohan V, Thakurdesai PA. Effect of glycosides based standardized fenugreek seed extract in bleomycin- induced pulmonary fibrosis in rats: decisive role of Bax, Nrf2, NF-KB, Muc5ac, TNF-α and IL-1β. Chem Biol Interact. 2015;237:151-165. DOI: 10.1016/j.cbi.2015.06.019.  Back to cited text no. 18
    
19.
Nikbakht J, Hemmati AA, Arzi A, Mansouri MT, Rezaie A, Ghafourian M. Protective effect of gallic acid against bleomycin-induced pulmonary fibrosis in rats. Pharmacol Rep. 2015;67(6):1061-1067. DOI: 10.1016/j.pharep.2015.03.012.  Back to cited text no. 19
    
20.
Sadeghi H, Jahanbazi F, Sadeghi H, Omidifar N, Alipoor B, Kokhdan EP, et al. Metformin attenuates oxidative stress and liver damage after bile duct ligation in rats. Res Pharm Sci. 2019;14(2):122-129. DOI: 10.4103/1735-5362.253359.  Back to cited text no. 20
    
21.
Arya A, Azarmehr N, Mansourian M, Doustimotlagh AH. Inactivation of the superoxide dismutase by malondialdehyde in the nonalcoholic fatty liver disease: a combined molecular docking approach to clinical studies. Arch Physiol Biochem. 2019:1-8. DOI: 10.1080/13813455.2019.1659827.  Back to cited text no. 21
    
22.
Mansourian M, Sadeghi H, Doustimotlagh AH. Activation of the glutathione peroxidase by metformin in the bile-duct ligation-induced liver injury: in vivo combined with molecular docking studies. Curr Pharm Des. 2018;24(27):3256-3263. DOI: 10.2174/1381612824666181003114108.  Back to cited text no. 22
    
23.
Ahluwalia N, Shea BS, Tager AM. New therapeutic targets in idiopathic pulmonary fibrosis. Aiming to rein in runaway wound-healing responses. Am J Respir Crit Care Med. 2014;190(8):867-878. DOI: 10.1164/rccm.201403-0509PP.  Back to cited text no. 23
    
24.
Guan R, Wang X, Zhao X, Song N, Zhu J, Wang J, et al. Emodin ameliorates bleomycin-induced pulmonary fibrosis in rats by suppressing epithelial- mesenchymal transition and fibroblast activation. Sci Rep. 2016;6:35696,1-14. DOI: 10.1038/srep35696.  Back to cited text no. 24
    
25.
Zaafan MA, Zaki HF, El-Brairy AI, Kenawy SA. Pyrrolidinedithiocarbamate attenuates bleomycin- induced pulmonary fibrosis in rats: modulation of oxidative stress, fibrosis, and inflammatory parameters. Exp Lung Res. 2016;42(8-10):408-416. DOI: 10.1080/01902148.2016.1244578.  Back to cited text no. 25
    
26.
Azarmehr N, Afshar P, Moradi M, Sadeghi H, Sadeghi H, Alipoor B, et al. Hepatoprotective and antioxidant activity of watercress extract on acetaminophen-induced hepatotoxicity in rats. Heliyon. 2019;5(7):e02072,1-5. DOI: 10.1016/j.heliyon.2019.e02072.  Back to cited text no. 26
    
27.
Nili-Ahmadabadi A, Alibolandi P, Ranjbar A, Mousavi L, Nili-Ahmadabadi H, Larki-Harchegani A, et al. Thymoquinone attenuates hepatotoxicity and oxidative damage caused by diazinon: an in vivo study. Res Pharm Sci. 2018;13(6):500-508. DOI: 10.4103/1735-5362.245962.  Back to cited text no. 27
    
28.
Fahlyani BK, Behzad-Behbahani A, Taghavi SA, Farhadi A, Salehi S, Adibzadeh S, et al. Development of an in-house taqman real time RT-PCR assay to quantify hepatitis C virus RNA in serum and peripheral blood mononuclear cells in patients with chronic hepatitis C virus infection. Hepat Mon. 2015;15(8):e28895,1-6. DOI: 10.5812/hepatmon.28895.  Back to cited text no. 28
    
29.
El-Khouly D, El-Bakly WM, Awad AS, El- Mesallamy HO, El-Demerdash E. Thymoquinone blocks lung injury and fibrosis by attenuating bleomycin-induced oxidative stress and activation of nuclear factor Kappa-B in rats. Toxicology. 2012;302(2-3):106-113. DOI: 10.1016/j.tox.2012.09.001.  Back to cited text no. 29
    
30.
Coward WR, Saini G, Jenkins G. The pathogenesis of idiopathic pulmonary fibrosis. Ther Adv Respir Dis. 2010;4(6):367-388. DOI: 10.1177/1753465810379801.  Back to cited text no. 30
    
31.
Soskic SS, Dobutovic BD, Sudar EM, Obradovic MM, Nikolic DM, Djordjevic JD, et al. Regulation of inducible nitric oxide synthase (iNOS) and its potential role in insulin resistance, diabetes and heart failure. Open Cardiovasc Med J. 2011;5:153-163. DOI: 10.2174/1874192401105010153.  Back to cited text no. 31
    
32.
Abuelezz SA, Hendawy N, Osman WM. Aliskiren attenuates bleomycin-induced pulmonary fibrosis in rats: focus on oxidative stress, advanced glycation end products, and matrix metalloproteinase-9. N-S Arch Pharmacol. 2016;389:897-909. DOI: 10.1007/s00210-016-1253-3.  Back to cited text no. 32
    
33.
Kalayarasan S, Sriram N, Sudhandiran G. Diallyl sulfide attenuates bleomycin-induced pulmonary fibrosis: critical role of iNOS, NF-κB, TNF-α and IL- ip. Life Sci. 2008;82(23-24):1142-1153. DOI: 10.1016/j.lfs.2008.03.018.  Back to cited text no. 33
    
34.
Bahramikia S, Yazdanparast R. Antioxidant efficacy of Nasturtium officinale extracts using various in vitro assay systems. J Acupunct Meridian Stud. 2010;3(4):283-290. DOI: 10.1016/S2005-2901(10)60049-0.  Back to cited text no. 34
    
35.
Del Rio D, Stewart AJ, Pellegrini N. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis. 2005;15(4):316-328. DOI: 10.1016/j.numecd.2005.05.003.  Back to cited text no. 35
    
36.
Boligon AA, Janovik V, Boligon AA, Pivetta CR, Pereira RP, Rocha JBTd, et al. HPLC analysis of polyphenolic compounds and antioxidant activity in Nasturtium officinale. Int J Food Prop. 2013;16(1):61-69. DOI: 10.1080/10942912.2010.528111.  Back to cited text no. 36
    
37.
Shahani S, Behzadfar F, Jahani D, Ghasemi M, Shaki F. Antioxidant and anti-inflammatory effects of Nasturtium officinale involved in attenuation of gentamicin-induced nephrotoxicity. Toxicol Mech Methods. 2017;27(2):107-114. DOI: 10.1080/15376516.2016.1258748.  Back to cited text no. 37
    


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4]
 
 
    Tables

  [Table 1]



 

Top
 
 
  Search
 
Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
Access Statistics
Email Alert *
Add to My List *
* Registration required (free)

 
  In this article
   Abstract
    Introduction
    Material and Methods
    Results
    Discussion
    Conclusion
   References
   Article Figures
   Article Tables

 Article Access Statistics
    Viewed414    
    Printed0    
    Emailed0    
    PDF Downloaded34    
    Comments [Add]    

Recommend this journal


[TAG2]
[TAG3]
[TAG4]