Research in Pharmaceutical Sciences

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 11  |  Issue : 6  |  Page : 470--475

Lovastatin prevents bleomycin-induced DNA damage to HepG2 cells


Marjan Nasiri, Mahmoud Etebari, Abbas Jafarian-Dehkordi, Shahla Moradi 
 Department of Pharmacology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran

Correspondence Address:
Mahmoud Etebari
Department of Pharmacology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan
I.R. Iran

Lovastatin as a member of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitors is used as a lipid-lowering agent. It can also inhibit the formation of hydrogen peroxide and superoxide anion and finally leads to decline in oxidative stress processes. Here, we evaluated whether lovastatin can increase DNA damage resistance of HepG2 cells against genotoxicity of the anticancer drug bleomycin (BLM). HepG2 cells were incubated with different concentrations of lovastatin (0.1, 0.5, 1, 5 µM) before exposure to BLM (0.5 µg/mL for one h). The genotoxic dose of BLM and lovastatin was separately determined and comet assay was used to evaluate the genotoxicity. After trapping cells in agarose coated lames, they were lysed and the electrophoresis was done in alkaline pH, then colored and monitored by florescent microscope. The results of this study indicated that lovastatin in doses lower than 5 µM has genoprotective effect and in doses higher than 50 µM is genotoxic. In conclusion, lovastatin is able to protect genotoxic effects of BLM in HepG2 cells. Further studies are needed to elucidate the mechanism(s) involved in this process.


How to cite this article:
Nasiri M, Etebari M, Jafarian-Dehkordi A, Moradi S. Lovastatin prevents bleomycin-induced DNA damage to HepG2 cells.Res Pharma Sci 2016;11:470-475


How to cite this URL:
Nasiri M, Etebari M, Jafarian-Dehkordi A, Moradi S. Lovastatin prevents bleomycin-induced DNA damage to HepG2 cells. Res Pharma Sci [serial online] 2016 [cited 2021 Jan 27 ];11:470-475
Available from: https://www.rpsjournal.net/article.asp?issn=1735-5362;year=2016;volume=11;issue=6;spage=470;epage=475;aulast=Nasiri;type=0