Research in Pharmaceutical Sciences

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 15  |  Issue : 6  |  Page : 529--540

A simple and validated HPLC method for vancomycin assay in plasma samples: the necessity of TDM center development in Southern Iran


Parisa Ghasemiyeh1, Afsaneh Vazin2, Farid Zand3, Amir Azadi5, Iman Karimzadeh2, Soliman Mohammadi-Samani4 
1 Department of Clinical Pharmacy; Department of Pharmaceutics, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, I.R. Iran
2 Department of Clinical Pharmacy, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, I.R. Iran
3 Anesthesiology and Critical Care Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, I.R. Iran
4 Department of Pharmaceutics; Center for Nanotechnology in Drug delivery, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, I.R. Iran

Correspondence Address:
Soliman Mohammadi-Samani
Department of Pharmaceutics; Center for Nanotechnology in Drug delivery, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz
I.R. Iran

Background and purpose: Vancomycin is a glycopeptide antibiotic which is the drug of choice against methicillin-resistant Staphylococcus aureus. It has a narrow therapeutic index, and thus therapeutic drug monitoring (TDM), and clinical pharmacokinetic assessment are necessary in order to prevent adverse drug reactions such as nephrotoxicity. In this study, we aimed to develop a simple and validated HPLC method for vancomycin assay in order to establish a TDM center for patients admitted to the ICU of Nemazee Hospital in southern Iran. Experimental approach: In this study, a brief review of different parameters and variables which could affect the sensitivity, selectivity of the validated HPLC method for vancomycin determination were considered. According to the previous studies a simple, fast, and the relatively low-cost method was established for vancomycin determination in plasma samples. Findings/Results: The developed HPLC assay indicated a calibration curve with R-square of > 0.999, acceptable selectivity, the accuracy of 90-105%, CV% of less than 15%, the limit of quantification of 1 μg/mL, and limit of detection of 300 ng/mL. Vancomycin trough level, the area under the curve, renal clearance, the volume of distribution,, and elimination constant were measured in patients using this validated method. Conclusion and implications: Validated method for assay of vancomycin plasma levels was used to quantify vancomycin levels of four patients who were admitted to the ICU of Nemazee Hospital. According to the results, two of these patients showed lower levels than recommended therapeutic purposes while one of them showed a toxic level. According to the results, the TDM assessment of vancomycin is strongly recommended for patients who are hospitalized in ICU.


How to cite this article:
Ghasemiyeh P, Vazin A, Zand F, Azadi A, Karimzadeh I, Mohammadi-Samani S. A simple and validated HPLC method for vancomycin assay in plasma samples: the necessity of TDM center development in Southern Iran.Res Pharma Sci 2020;15:529-540


How to cite this URL:
Ghasemiyeh P, Vazin A, Zand F, Azadi A, Karimzadeh I, Mohammadi-Samani S. A simple and validated HPLC method for vancomycin assay in plasma samples: the necessity of TDM center development in Southern Iran. Res Pharma Sci [serial online] 2020 [cited 2021 Oct 17 ];15:529-540
Available from: https://www.rpsjournal.net/article.asp?issn=1735-5362;year=2020;volume=15;issue=6;spage=529;epage=540;aulast=Ghasemiyeh;type=0