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  Indian J Med Microbiol
 

Figure 1: (a), Expression of reteplase. Lane 1: induced total bacterial protein; lane 2: uninduced total bacterial protein; and lane 3: protein marker; (b), Isolation of reteplase inclusion bodies. Lane 1: inclusion bodies pellet; lane 2: total protein after induction; lane 3: protein marker; lane 4: supernatant after cell disruption; and lane 5-7: supernatants after 1-3 washing of inclusion bodies pellet.

Figure 1: (a), Expression of reteplase. Lane 1: induced total bacterial protein; lane 2: uninduced total bacterial protein; and lane 3: protein marker; (b), Isolation of reteplase inclusion bodies. Lane 1: inclusion bodies pellet; lane 2: total protein after induction; lane 3: protein marker; lane 4: supernatant after cell disruption; and lane 5-7: supernatants after 1-3 washing of inclusion bodies pellet.