Figure 2: Up regulation of tumor necrosis factor-α (TNF-α) level in HUVECs by T4 exposure. After treatments with T4 (50, 100, and 200 nmol/L) for 12, 24, and 48 h, protein expression evaluated by ELISA method. *P < 0.001 indicates significant differences compared with control group. Data are presented as mean ± SD. Values derived from three independent experiments.