Figure 7: Protein detection for evaluation of ICAM-1 expression performed using western blotting technique. (A) Protein expression levels of ICAM-1 in HUVECs cells treated by T4 (50, 100, and 200 nmol/L) for 24 and 48 h and (B) T4 treatment significantly increased relative expression of ICAM-1 compared with control group. For analysis protein's bands densities, Image J software was used to analyze the densities. Each protein bands normalized to β-actin bands. ICAM-1, Intercellular adhesion molecule 1.